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levE [2018-07-26 14:52:28]
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levE [2018-07-26 14:52:28]

trigger enzyme: fructose-specific phosphotransferase system, EIIB component of the PTS
locus
BSU27060
pI
9.44
mw
17.94 kDa
protein length
162 aa Sequence Blast
gene length
486 bp Sequence Blast
function
fructose uptake and phosphorylation, control of LevR activity
product
trigger enzyme: fructose-specific phosphotransferase system, EIIB component
essential
no
ec
2.7.1.69
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.7|Utilization of fructose]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    2,761,907 → 2,762,395

    The protein

    Catalyzed reaction/ biological activity

  • Protein EIIB N(pi)-phospho-L-histidine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

    • Protein family

  • [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, mannose permease (Man) family [Pubmed|10627040]

    • Structure

  • [PDB|1BLE]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]-[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]-[gene|8ABA3735067C7AA433E09F219EE7239196377A95|levF]-[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]-[gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]
    description
    [Pubmed|2117666]

    sigma factors

  • [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [Pubmed|1924373], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7592486], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|1D2043CC0D8CF64142F0A5993A936C5A196726D4|LevR]: activation, [Pubmed|1900939], in [regulon|1D2043CC0D8CF64142F0A5993A936C5A196726D4|LevR regulon]

    • regulation

  • induced in the presence of fructose ([protein|search|LevR]) [Pubmed|1900939]
    • view in new tab

    Biological materials

    Mutant

  • BKE27060 (Δ[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATAATTCATCCTCTCT, downstream forward: _UP4_ACAAAATAATCAAGGGGATG
  • BKK27060 (Δ[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATAATTCATCCTCTCT, downstream forward: _UP4_ACAAAATAATCAAGGGGATG

    • References

  • 10627040,2117666,9551099,9030753,9033408,1924373,7592486,1900939